Fig. 6
Palbociclib-resistant and cisplatin-resistant NPC cells remain sensitive to cisplatin and palbociclib, respectively. a. Western blot analysis of the cell cycle-related genes E-cadherin and N-cadherin in parental and PD-resistant NPC43 cell lines in the presence or absence of palbociclib treatment (5 μM). b. The cancer stem cell–related gene expression profiles in parental and palbociclib-resistant (PD_R) cells were evaluated by qPCR. c. A colony formation assay verified the resistance of NPC43 PD_R cells to palbociclib. d. Determination of the concentrations that would induce a 50% inhibition of colony formation (ICol50) in both the parental and pablbociclib-resistant NPC43 and C666–1 cell lines. e. The sensitivity of NPC43 PD_R and C666–1 PD_R cells to cisplatin was compared to that of the respective parental cell lines through a resazurin cell viability assay. f. Cisplatin resistance in NPC43 sublines #5 and #5 N was verified through a resazurin cell viability assay. g. The sensitivity of parental and cisplatin-resistant NPC43 cells to palbociclib was tested through a resazurin cell viability assay