Fig. 6

GOLPH3L interacts with SERPINE1 by stabilizing its expression and promotes its tumorigenic activity. a GOLPH3L was confirmed to interact with SERPINE1 in T47D and BT474 cells with a Co-IP assay. b The knockdown of GOLPH3L in T47D cells reduced the stability of SERPINE1, while overexpressing GOLPH3L stabilized SERPINE1; p-values< 0.05 are indicated. c Expression of GOLPH3L was correlated with the ubiquitination levels of SERPINE1. T47D cells with GOLPH3L siRNA and/or overexpression were subjected to immunoprecipitation and Western blot analyses. d The overexpression of SERPINE1 partially reversed the proliferative defects induced by GOLPH3L suppression. The cell number was determined with CCK-8 assay, n = 3. * p < 0.05, ** p < 0.01. e SERPINE1 expression rescued the inhibition of tumorigenesis caused by GOLPH3L suppression. Representative images of colonies revealed by crystal violet staining and quantification of colony numbers of each group, n = 3. * p < 0.05, ** p < 0.01