Fig. 6

DNMT3a activates JAK2/STAT3 signaling pathway by suppressing SOCS3 expression. a Western blot results of genes involved in JAK/STAT3 signaling pathway after deletion of DNMT3a. b PGL3-SOCS3 promoter was co-transfected with different doses of MYC-DNMT3a, the activity of SOCS3 promoter was measured by luciferase. Renilla was used as control. c Diagram of CpG island in SOCS3 promoter. d ChIP-qPCR results of DNMT3a on SOCS3 promoter and its nearby non-CpG region. IgG was used as control. e Detection of SOCS3 promoter methylation with Methylated-DNA IP Kit. f Detection of SOCS3 promoter methylation after depleting DNMT3a. *P < 0.05, **P < 0.01, ***P < 0.001