Fig. 5

Identification of genome-wide DNA binding sites and transcription targets for ZNF703. a ChIP Peaks over chromosomes by ChIP-seq using the primary antibody against ZNF703 (The abscissa represents the length of the chromosome, the right represents the chromosome number, and the left ordinate represents the peak value of each chromosome). b The distribution of reads on both sides of the transcription start site (TSS). c Pie diagram showed the ratios of ZNF703 binding sites located relative to a transcription unit including intergenic, 1st exon, 1st intron, TTS, promoter, other intron and other exon. d GO enrichment of Peak related genes (top 20 terms). e KEGG enrichment map of metabolic pathways of Peak-related genes (top 20 terms). f Five common motifs with the most significant differences among peaks. g Interaction network diagram between TFs and genes based on sequencing results, purple represented the transcription factor or transcription factor family, red represented the target gene that TF may act through motifs. h ChIP-PCR to detect the binding of ZNF703 on the enhancer of PEA15 in CAOV3 and OVCAR3 cells using ZNF703 primary antibody, IgG was used as a negative control. The experiment was repeated three times. i, ChIP-PCR products were analyzed in OVCAR3 cell using horizontal agarose gel electrophoresis and visualized using UV. Data are presented as mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001