Fig. 5

KLF7 silencing reduces HGSOC spheroid size. a, c Spheroid assay results using OV-90 and PEO1 cells transfected with siKLF7 and with siC, as control, after 10 days of culture. Left panel: representative bright field microscopy (20x; scale bars, 100 μm) images of transfected OV-90 and PEO1 spheroids released from the hydrogel. Right panel: bar chart showing spheroid size (μm), measured by Leica Application Suite (LAS) analysis. Bars and error bars refer to mean and SD (Standard Deviation) of three experiments. At least 20 spheroids/condition were analysed for each experiment. b, d Representative immunofluorescence (100x; scale bars, 20 μm) images of transfected OV-90 and PEO1 spheroids (siC and siKLF7) released from the hydrogel. The images show the merged signal of Alexa Fluor 488-Vimentin (VIM) mouse monoclonal antibody (green) and DAPI (40,6-diamidino-2-phenylindole) (blue). To establish statistically significant differences, unpaired t-test was carried out: **p < 0.01