Fig. 4

Effect of JVG045 on human neuroblastoma cell lines. a Dose response of JVG045 on human neuroblastoma cell lines. The graph shows that the tricarbonyl Rhenium compound has very little effect on cell number in those cell lines with non-amplified MYCN (SHSY5 and hNB), while it has a significant effect in MYCN-amplified cell lines (Kelly, IMR-32 and LAN-1). b Representative western blot showing the effect of JVG045 (10 μM) on the phosphorylation of FGFR1(Y653/654) and Src(Y416) compared to the FGFR inhibitor BGJ398 and the Src inhibitor Bosutinib, respectively. ReCl was used as a negative control. Data from R2: Genomics Analysis and Visualization Platform (http://r2.amc.nl) databases illustrating the prognostic value of FGFR1 and Src in Pancreatic Ductal Adenocarcinoma (PDAC) (a and e), and Neuroblastoma (d and f). Results are showed as Mean ± SEM and experiments were performed in triplicate