Fig. 5

Circ-LRIG3 is regulated by p-STAT3. a qRT-PCR analysis of circ-LRIG3 in HepG2 and SMMC-7721 cells treated with colivelin and C188–9. b The STAT3 binding motif on circ-LRIG3 promoter predicted by JASPAR online tool. c The designed primers targeting the STAT3 binding sites on circ-LRIG3 promoter used for ChIP assay. d, e ChIP assay using anti-p-STAT3 antibody, followed by PCR analysis of enrichment. Anti-IgG antibody was used as a negative control. f The sketch showing the pGL3-basic reporter containing full-length circ-LRIG3 promoter sequence with wild-type or mutated STAT3 binding motif. g Luciferase reporter assay in HepG2 and SMMC-7721 cells transfected with above wild-type or mutated pGL3-basic reporter. **P < 0.01