Fig. 2
From: Choline kinase alpha impairment overcomes TRAIL resistance in ovarian cancer cells
TRAIL resistance in OC cell lines is not related to basal TRAIL-R2 expression levels and is specifically overcome by siCHKA. a. Representative flow cytometry analysis showing surface membrane expression of TRAIL-R2 in wild type OC cell lines. Fluorescence index is reported. b. Percentage of TRAIL-mediated growth inhibition of wild type OC cell lines following 16 h treatment with sTRAIL as compared to relative untreated cells. c. Correlative plot of TRAIL-R2 membrane basal expression (fluorescence index) versus percentage of TRAIL-mediated cell growth inhibition. In red are indicated TRAIL-resistant cell lines. d. OC cells sensitivity to sTRAIL treatment following: siCHKA; recovery of ChoKα expression (144 h after silencing); siCHKB; deprivation of methionine (−Met) and deprivation of glutamine (−Gln). Asterisks are referred to statistically significant differences. Dotted lines indicated 50% growth inhibition