Fig. 2

Knockdown of MIEF2 suppressed OC cell growth through induction of G1-S cell cycle arrest and cell apoptosis. a and b Knockdown of MIEF2 was confirmed by qRT-PCR and Western blot analysis in OVCAR3 and ES2 cells. siMIEF2, siRNA against MIEF2; siCtrl, control siRNA. c and d MTS cell viability and colony formation assays in OVCAR3 and ES2 cells with or without MIEF2 knockdown. e EdU incorporation assay was performed in OVCAR3 and ES2 cells with or without MIEF2 knockdown. Scale bars, 50 μm. f and g Flow cytometry analysis for cell cycle distribution and apoptosis in OVCAR3 and ES2 cells with or without MIEF2 knockdown. *P < 0.05