Fig. 5

Knockdown of BACE2 in MES GSCs restrains stemness and radioresistance. a Western blotting analysis for MES-related signatures CD44, YKL40 and pathways p65, p-p65 and C/EBPβ after silencing BACE2 in GSC20 and 267. b Left, images of spheres formation assay after GSC20 and 267 transfected with lentiviral shBACE2 or shNT and right, results were quantified with diameter of tumor spheres. Scale bar, 500 μm. c In vitro limiting dilution assay for GSC20 and 267 expressing lentiviral shBACE2 or shNT. d Cell cycle analysis for G2/M phase cells of GSC20 and 267 in control, 3 Gy radiation only or combined with shBACE2 groups. e Flowcytometry detected apoptosis of GSC20 and 267 in control, 3 Gy radiation only or combined with shBACE2 groups. f Bioluminescence imaging for xenografts grouped as control, shBACE2 and/or radiation at the indicated time points. The radiation was delivered at day 7 after GSCs implantation. g Survival curve for each group, and the statistical significance was tested by log-rank test. h Represented images of H&E staining in each group. The magnification was marked in blue number. Error bar indicates at least three independent experiments and data are shown as mean ± SD. *p < 0.05, **p < 0.01, ***P < 0.001