Fig. 2

MiR-375 regulates cell viability, cell migration and cell invasion in glioma cell lines. a. MiR-375 overexpression in U87 and U251 cells were validated by qRT-PCR. b, c. CCK-8 and EdU assays show that miR-375 upregulation inhibits cell proliferation in both U87 and U251 cells. Scale bar = 100 μm. d. Cell migration was determined by a wound healing assay. The migration distance was measured at 0, 24, and 48 h after the cells were scratched. Scale bar, 400 μm. e. The effect of miR-375 overexpression on the migration (left) and invasion (right) of both U87 and U251 cells, as examined using a transwell assay. Scale bar = 100 μm. All experiments were repeated independently three times. Data are presented as mean ± standard deviation. **p < 0.01; ***p < 0.001. ns, not significant