Fig. 5

Glioma cell-derived exosomes carry a high expression of miR-375. a. Immunoblotting for exosomal markers ALIX, TSG101 and CD9, as well as negative control, calnexin. b. Uranyl acetate negative stained TEM images, of exosomes isolated from U87 (left) and U251 cell lines (right). Scale bar = 200 nm. c. Size distribution of the isolated exosomes was analysed by Zetasizer Nano-Zs. d. Detection of miR-375 relative expression levels in U87 and U251 cells and their derived exosomes by qRT-PCR. e. Detection of miR-375 relative expression levels in miR-375 overexpressing U87 and U251 cells derived exosomes by qRT-PCR. All experiments were repeated independently three times. Data are presented as mean ± standard deviation. **p < 0.01; ***p < 0.001. ns, not significant