Fig. 7

Exosome miR-375 secreted by glioma cells is less efficiently taken up by glioma cells. a. Exosomes labelled with PKH26 and incubated with U87/U251 cells for 24 h. Representative laser confocal images showing exosomes merged with U87 (upper panel) and U251 (lower panel). Exosomes are labelled with PKH26 (red), and glioma cells are labelled with GFP (green). Nuclei stained with DAPI (blue). Scale bar = 10 μm. b. Fluorescence images of miR-375 mimic-EXO incubated with glioma cells for 24 h. Exosomes are labelled with PKH26 (red). Nuclei stained with DAPI (blue). Scale bar = 100 μm. c. The uptake of exosomes by glioma cells was assessed by flow cytometry. d. CCK-8 analysis showing the effect of miR-375 mimic-EXO on the proliferation of U87 and U251 cells. e. Wound healing analysis demonstrating the effect of miR-375 mimic-EXO on the migration of U87 and U251 cells. Scale bar = 400 μm. f. Transwell analysis demonstrating the effect of miR-375 mimic-EXO on glioma cell migration (left) and invasion (right). Scale bar = 100 μm. g. qRT-PCR analysis of the effect of miR-375 mimic-EXO on the miR-375 content in U87 and U251 cells. All experiments were repeated independently three times. Data are presented as mean ± standard deviation. *p < 0.05; **p < 0.01. ns, not significant