Fig. 7

Let-7a-regulated cancer cell OXPHOS is dependent on SNAP23. a Staining of SW480 cells treated with ctrl or let-7a inhibitor with Mito-tracker (red) and anti-SNAP23 (green). Scale bar, 10 μm. b, c SW480 sh-NT and sh-SNAP23 cells were transiently transfected with ctrl or let-7a inhibitor, then subjected to measure basal OCR and SRC using Seahorse analyzer. d Western blot analysis of indicated protein expression of SW480 sh-NT and sh-SNAP23 cells treated with ctrl or let-7a inhibitor. e Cellular ATP levels were measured in SW480 sh-NT and sh-SNAP23 cells treated with ctrl or let-7a inhibitor. f qPCR analysis of indicated genes in SW480 cells overexpressing Vector and SNAP23. g Western blot analysis of Lin28a and SDHA protein expression in SW480 cells overexpressing Vector and SNAP23. h Western blot analysis of Lin28a and SDHA in SW480 cells overexpressing Vector and SNAP23 transiently transfected with siNTC or siLin28a. i Model for the role of let-7a in the EV secretion and mitochondrial OXPHOS via SNAP23 in CRC. Data represent the mean ± SEM of at least three independent experiments. *p < 0.05, and **p < 0.01