Fig. 7

(a) Necrosis (relative LDH release), (b) cell protein and (c) apoptosis (relative caspase-3 activity) of AT1 and Walker-256 cells in vitro after 24 h at pH 7.4 and 6.6. (d) Apoptosis in AT1 and Walker-256 tumors in vivo 24 h after inducing metabolic acidosis by intensifying glycolytic metabolism. n = 9–15; (*) p < 0.05, (**) p < 0.01 acidosis vs. control