Fig. 5

PKM2, HIF-1α and PPAR-γ may be involved in Sora and Sim co-treatment effects. (a) qPCR results of 18 genes associated with glycolysis, glucose metabolism and fatty acid metabolism. (b) Western blotting analysis of HIF-1α, PPAR-α and PPAR-γ in LM3-SR cells. (c) The IF staining of PKM2, HIF-1α and PPAR-γ in LM3-SR cells. (d) A cytoplasm-nuclear protein extraction kit used to analyze the distribution of PKM2, HIF-1α and PPAR-γ in LM3-SR cells. LaminA/C and β-tubulin were used as internal references for the nucleus and cytoplasm, respectively. (e) Co-IP assay used to determine the interaction between PKM2, HIF-1α and PPAR-γ