Fig. 2

High expression of exosomal circRNA-100,338 affects HCC cell invasion. a qRT-PCR analysis of circRNA-100,338 expression in HCC cells (Hep3B and MHCC97H) and in their secreted exosomes (Hep3B-exo and MHCC97H-exo). b qRT-PCR analysis of exosomal circRNA-100,338 expression in a series of HCC cell lines with distinct metastatic potential, including HLE, Huh7, Hep3B, BEL7402, SMMC7721, MHCC97L, MHCC97H, HCCLM3, and HCCLM6, and a normal liver cell line, L02. c The exosomes derived from circRNA-100,338 overexpressing (CIRC-exo) MHCC97H cells promoted the invasive ability of MHCC97L, SMMC7721, BEL7402, Hep3B, Huh7, and HLE cells. d The exosomes derived from circRNA-100,338 knockdown (siCIRC) MHCC97H cells suppressed the invasive ability of MHCC97L, SMMC7721, BEL7402, Hep3B, Huh7, and HLE cells. The control exosomes for CIRC-exo and siCIRC were labeled as Scramble-exo and siNC-exo. e Gelatin zymography assay showed the activity of MMP9 and MMP2 in Hep3B after treated with the exosomes derived from MHCC97H. f-g ELISA assay showed that exosomes derived from circRNA-100,338 overexpressing (CIRC-exo) or knockdown (siCIRC) MHCC97H cells significantly increased (f) or decreased (g) the expression levels of MMP9 in MHCC97L, SMMC7721, BEL7402, Hep3B, Huh7, and HLE cells. Significance was defined as P < .05 (*P < .05; **P < .01; ***P < .001)