Fig. 3

Exosomal circRNA-100,338 regulates HUVEC cell proliferation, angiogenesis, and permeability. a-b HUVECs cells after 3 h incubation of exosomes isolated from Hep3B and MHCC97H cells with fluorescently labeled PKH26. Red represents exosomes staining by PKH26, and blue represents nuclear DNA staining by DAPI. c qRT-PCR analysis of circRNA-100,338 expression in HUVEC cells after treated with exosomes derived from circRNA-100,338 overexpressing Hep3B (Hep3B-CIRC-exo) or knockdown MHCC97H (MHCC97H-siCIRC-exo) cells, controls of which were labeled as Hep3B-scramble-exo and MHCC97H-siNC-exo. d-e CCK-8 assay detected the proliferation rate of HUVEC after treated with exosomes derived from circRNA-100,338 knockdown MHCC97H cells (d) and overexpressing Hep3B cells (CIRC-exo) (E). f Tube formation of HUVECs after treated with exosomes derived from circRNA-100,338 knockdown MHCC97H cells (siCIRC) and overexpressing Hep3B cells (CIRC). g Transwell assay was used to detect the effects of exosomal circRNA-100,338 on the ability to migrate HUVEC cells. h Histogram plot showed the number of migrated cells. i Moreover, exosomes derived from circRNA-100,338 knockdown MHCC97H cells (siCIRC-exo) and overexpressing Hep3B cells (CIRC-exo) affected the permeability of HUVEC monolayers. Significance was defined as P < .05 (*P < .05; **P < .01; ***P < .001)