Fig. 1

miR-506-3p down-regulated MYCN expression. Cells were transfected with 25 nM miR-506-3p mimic or control oligo for 2 days, and RNAs and protein lysates were harvested. a MYCN mRNA levels as measured by qPCR. Shown are the relative expression level of MYCN mRNA in each cell line transfected with the miR-506-3p mimic normalized to that in the same cell line transfected with control oligo. *, p < 0.05 comparing to control. b Representative Western blots of N-Myc protein levels, with Calnexin protein levels measured as a loading control. Values shown above the N-Myc bands are the calculated relative intensities of the corresponding bands