Fig. 4

TMEM52B suppression promotes invasion and cell survival in an EGFR-dependent manner. (A) Immunoblot analysis of EGFR phosphorylation in cells transfected with shRNA specific to TMEM52B. (B) Cells were transfected with shRNA specific to TMEM52B for 48 h. Transfected cells were allowed to invade Matrigel in the absence or presence of EGF (10 ng/ml) for 48 h. Cell invasion was determined by calculating the cell-stained area relative to the total area using ImageJ software. (C-E) Cells were co-transfected with siRNA specific to EGFR, and shRNA specific to TMEM52B for 48. (C) Transfected cells were lysed for immunoblot analysis. Densitometric quantification was performed on the immunoblots using GAPDH as a loading control. Transfected cells were subjected to invasion (D) and cell survival (E) assays as described in Fig. 2A and B. All determinations were performed in three independent experiments. Values represent mean ± SD. *P < 0.05 compared with shControl + siControl; ^§P < 0.05 compared with shTMEM52B + siControl