Fig. 5

5’tiRNA-His-GTG is involved in the hippo signaling pathway by regulating LATS2 expression. a Schematic diagram of 5’tiRNA-His-GTG target prediction. b Experimental validation of the 10 highest-scoring 5’tiRNA-His-GTG target genes using qRT-PCR. c Western blot of LATS2, phospho-YAP (Ser127), and YAP expression upon 5’tiRNA-His-GTG mimic or inhibitor transfection in the indicated CRC cells. d Experimental validation of downstream genes of hippo signaling pathway using qRT-PCR. e Schematic diagram of the predicted interaction position between 5’tiRNA-His-GTG and the seed regions within the 3’UTR region and mutation region of LATS2. f The luciferase activity of pmirGLO-LATS2 was significantly decreased by the 5’tiRNA-His-GTG mimic in HCT116 cells. g HCT116 cells were co-transfected with a plasmid expressing the LATS2 3’UTR, 5’tiRNA-His-GTG mimic, and siRNAs targeting AGO1, AGO2, AGO3, and AGO4. After 48 h, transfected cells were collected with the luciferase assay. *p < 0.05, **p < 0.01, ns: not significant. All data are representative of at least three independent experiments and are presented as the means ± SD. LATS2, large tumor suppressor kinase 2; qRT-PCR, quantitative real-time reverse transcription PCR; YAP, Yes-associated protein; CRC, colorectal cancer; UTR, untranslated region; AGO, argonaute