Fig. 1

Timeline of in vivo GKT771 studies in DEN-induced HCC. a In the preventive study, five-week-old male SV129 wild-type received intraperitoneal injections of saline or 35 mg/kg DEN for 25 weeks to induce HCC. During HCC development (week 15 of DEN), mice were treated daily with vehicle or GKT771 (30 mg/kg) via oral gavage for 15 weeks. b In the therapeutic studies, mice received weekly DEN injections for 25 weeks or 20 week in the early and delayed treatment regimen, respectively. In the early treatment regimen, mice were treated with GKT771 twice daily after 20 weeks of DEN for a total of 9 weeks (early euthanasia). Mice in the delayed treatment regimen were kept in a resting period of 2 weeks after the final DEN injection prior to daily GKT771 treatment for 5 weeks