Fig. 1
Antitumor therapeutic effect of the anti-16E6 scFvI7nuc and anti-16E7 scFv43M2SD on TC-1-Luc tumors. The graph shows the luminescent signal quantification of pooled data from tumors developed by injection of 5 × 104 TC-1-LUC cells in C57/BL6 mice. The scFvI7nuc (I7nuc) and scFv43M2SD (M2SD), and the irrelevant anti-β-galactosidase scFvR4nuc (R4nuc) and scFvR4sec (R4sec) as negative controls, were delivered to tumors of randomized groups of mice, four times at one-week interval. Tumor growth was monitored with the IVIS® Lumina imaging system. Photon emission was measured in specific regions of interest (ROI) and expressed as photon/second/cm2/steradian (p/s/cm2/sr). The difference between the mean values of photon emission of therapeutic scFvs versus their controls was statistically significant (p = 0.0195 for I7 nuc, p = 0.049 for 43M2SD) as calculated at T5. T is the time point in weeks after tumor cell challenge