Fig. 6

The LINC00680-miR-568-AKT3 axis regulates stemness properties and chemosensitivity in HCC cells. a. Expression of AKT3 in SNU-449 cells and SK-hep1 co-transfected with miR-568 inhibitors or miR-568 inhibitors NC and sh-LINC00680 or sh-LINC00680 NC. b. Expression of stemness markers in SNU-449 cells co-transfected with miR-568 inhibitor or miR-568 inhibitor NC and Sh-LINC00680 or sh-LINC00680 NC (left); Expression of cellular stemness biomarkers in SNU-449 co-transfected with Sh-LINC00680 or sh-LINC00680 NC and AKT3 or AKT3-vec (right). c. Sphere-forming capacities of SNU-449 cells co-transfected with miR-568 inhibitor or miR-568 inhibitor NC and sh-LINC00680 or Sh-LINC00680 NC (left); Sphere-forming capacities of SNU-449 cells co-transfected with sh-LINC00680 or sh-LINC00680 NC and AKT3 or AKT3-vec. (right) d. Cell viability analysis for SNU-449 cells co-transfected with Sh-LINC00680 or Sh-LINC00680 NC and miR-568 inhibitor or miR-568 inhibitor NC following treatment by different concentrations of 5-Fu (left); Cell viability analysis for SNU-449 cells co-transfected with sh-LINC00680 or sh-LINC00680 NC and AKT3 or AKT3-vec following treatment by different concentrations of 5-Fu (right). e. Expression of total and phosphorylated AKT3, mTOR, eIF4ebp1, p70S6K following manipulation of LINC00680 in HCC cell lines. ^**P < 0.01