Fig. 4

HOXA10 modulated the expression of EMT-related molecules in GC cells. a-d Expression of EMT-related molecules (E-cadherin, N-cadherin, Vimentin, Snail, Slug) in indicated groups of AGS and BGC823 cells were analyzed by western blot (a-b) and qRT-PCR (c-d). e Representative confocal Immunofluorescence staining of E-cadherin, N-cadherin and Vimentin in different GC cells with modified HOXA10 expression. f Representative IHC staining of HOXA10, E-cadherin, N-cadherin and Vimentin in GC cancer tissues with or without LNM. Immunofluorescence scale bars: 25 μm. IHC original magnification, 100×. **P < 0.01, ***P < 0.001