Fig. 8

HOXA10 regulated EMT progression partly through modulating METTL3. a-b qRT-PCR assays and correlation analysis revealed an evident correlation between HOXA10 and METTL3 mRNA expression in 80 paired GC tissues (r = 0.433, P < 0.001). c-d Western blot showed the protein levels of EMT-related proteins in AGS-Ctrl and AGS-HOXA10 cells transfected with lentiviruses carrying sh-NC/sh-METTL3 or in BGC823-Ctrl and BGC823-shHOXA10 cells transfected with Vector/METTL3. e-f IHC analysis demonstrated that the lung metastasis burden caused by AGS-HOXA10 cells were alleviated with reduced staining by inhibiting METTL3. In contrast, the fewer lung colonized nodules induced by HOXA10 knockdown in BGC823-shHOXA110 cells were partly elevated with enhanced staining after overexpressing METTL3. Original magnification, 100 ×