Fig. 7

CCDC183-AS1 promotes HCC progression through the CCDC183-AS1/miR-589-5p/SKP1 axis. a-b. Relative protein levels of SKP1 were detected in HLF and 97H cells transfected with indicated NC, siCCDC183-AS1, miR-589-5p inhibitor or SKP1 using Western blot, respectively. c-d. CCK-8 assays were performed to determine the ability of proliferation in HLF and 97H cells transfected with indicated NC, siCCDC183-AS1, miR-589-5p inhibitor or SKP1, respectively. e-f. Cell migratory and invasive capabilities were assessed by transwell assays in HLF and 97H cells transfected with indicated NC, siCCDC183-AS1, miR-589-5p inhibitor or SKP1, respectively. g. Relative expression levels of CCDC183-AS1, miR-589-5p and SKP1 were measured in subcutaneous tumor tissues by qRT-PCR. h. Relative expression levels of SKP1 were observed in subcutaneous tumor tissues by IHC. i. Correlation between CCDC183-AS1 and SKP1 expression in paired HCC tissues (n=44). Data are presented as mean ± SD. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001; ns, no significance