Fig. 1

Spliceosome RNAi screen identifies novel splicing regulators of tumor cell proliferation. a Overview of steps used to select splicing factors involved in breast cancer cell proliferation. All assays were performed 72 h after transfection. b Correlation between SRB and nuclei count values (both proliferation measurements) in the primary screen for Hs578T (top) and MDA-MB-231 (bottom) cell lines. c Heatmap showing the Z-scores for splicing factor knockdown effects on proliferation in Hs578T and MDA-MB-231 72 h after knockdown. Splicing factors that upon knockdown inhibited proliferation in both assays and cell lines (highlighted clusters) were selected for further validation. d Nuclei counting Z-scores for all splicing factors in the primary screen. Factors selected for validation are highlighted in blue and red, respectively. e Examples of splicing factor knockdowns and nuclei counting. siKinasePool (siKP) was used as a negative control. Scale bar = 500 μm