Fig. 6

Effect of Pladienolide B treatment on breast cancer cell proliferation. a Correlation between CDCA5 and KI67, MCM2 and PCNA expression in 1097 primary breast tumors. b Log2 RNA expression levels of KI67, MCM2, PCNA and CDCA5 in ER positive (n = 807) and TNBC (n = 116) primary breast tumors. Statistical significance was determined using a Students’s t-test. *** p < 0.001. c Effect of 4 days PB treatment on proliferation of luminal, basal A or basal B cell lines. Mean + sd of 3 biological replicates. d PB treatment IC50 in luminal, basal A and basal B cell lines. e Effect of PB treatment (0.003uM) on MDA-MB-231 and Hs578T nuclear phenotype 24, 48 and 72 h after treatment. Scale bar = 50 um. f CDCA5 intron 1 retention after 48 h PB treatment. g CDCA5 intron 2 retention after 48 h PB treatment