Fig. 3

Overexpression of circAGAP1 promoted the proliferation, migration and invasion abilities of ccRCC cells. a, b Overexpression of circAGAP1 in ACHN and A498 as detected by RT-PCR. Data are presented as the means ± SEM from three independent experiments. c, d The viability of A498 and ACHN cells transfected with mock or circAGAP1 as indicated by the CCK-8 assay. e EdU analysis and quantification of EdU-positive A498 and ACHN cells transfected with mock or circAGAP1. Cells were subjected to hypoxia for 24 h. f Apoptotic rates of A498 and ACHN cells transfected with mock or circAGAP1 as detected by flow cytometry. Cells were subjected to hypoxia for 24 h. g, h Transwell assays of A498 and ACHN cells transfected with mock or circAGAP1. The experiments were repeated with at least three biological replicates. **p < 0.01, ***p < 0.001