Fig. 2

miR-34 is downregulated in head and neck cancer (HNSCC) and targets proto-oncogene MET. a Sequences of the mature miR-34a-5p and miR-34a-5p in humans and mice. b Bioinformatic analysis suggested direct interaction of both the miR-34a guide strand (hsa-miR-34a-5p) and the passenger strand (hsa-miR-34a-3p) the 3’UTR of MET mRNA. c through h: Expression of miR-34a-3p and miR-34a-3p were quantified by the TaqMan microRNA Assay in HNSCC tumor samples and matched controls (n = 42) (c and d), HNSCC and healthy plasma (e and f) and exosomes isolated from HNSCC patients and healthy subjects (n = 28) (g and h). 2^-ΔΔCT method was used for relative expression. Data was normalized to RNU48 or spike-in cel-mir-39. Data is presented as mean ± SD. * indicates p < 0.05