Fig. 10

ITPR3 knockdown attenuated the tumorigenicity of BCa in vivo. a Schematic diagram of the subcutaneously implanted tumor model in nude mice. b Tumors were obtained from nude mice in the shITPR3 group and shCon groups, and representative images are shown in the right panel. c The wet weights of tumors in the shITPR3 and shCon groups were measured and compared after the mice were sacrificed at the end of the experiment. Meanwhile, the tumor volume changes in the nude mice were measured every three days. d Protein expression levels of ITPR3, P-NF-κB, IKBa, Ki-67, and CD44 were detected by a western blot assay, and β-actin was used as an internal control. e Immunohistochemistry was used to analyze the expression of ITPR3, CD44, Ki-67 and IKBa. The scale bar represents 50 μm. ^*P < 0.05, ^**P < 0.01