Fig. 3

ITPR3 inhibition decreases BCa cell proliferation in vitro. a The efficiency of ITPR3 knockdown was verified by western blotting analysis in 5637 or 253 J cell lines transfected with ITPR3 shRNAs and shControl. b Cell viability was detected by an MTT assay with treatment as indicated. c An EdU incorporation assay for staining proliferating cells (red indicates EdU-incorporated cells; blue indicates nuclear staining with Hoechst 33342) was conducted in 5637 and 253 J cells with treatment as indicated. Scale bars, 100 μm. d The cell cycle distribution was analyzed by flow cytometry. Quantitative analysis of the results is shown below. e Cell cycle-related proteins were detected by western blotting using the indicated antibodies. The images show the changes in the proteins E2F1, P21, Cyclin D1, Cyclin B1 and CDK2 in 5637 and 253 J cells. β-actin was used as a loading control. ^*P < 0.05, ^**P < 0.01