Fig. 9

ITPR3 regulates EMT via the NF-ĸB/CD44 signaling pathway in BCa. a The migration and invasion abilities were analyzed by a transwell Boyden assay with or without Matrigel in 5637 and 253 J shITPR3 or shCon cells after treatment with TNFα or overexpression of CD44. Quantification analysis is shown below. b The schematic diagram of transwell assay. c A wound healing assay was also conducted as described above. Quantification analysis is shown on the right. d The gene expression of E-cadherin, N-cadherin, Vimentin and MMP2 was detected by a western blot assay in 5637 and 253 J cells after treatment as described above. β-actin served as an internal control. e The gene expression of CD44, OCT4, SOX2 and CDK2 was detected by a western blot assay in 5637 and 253 J cells after treatment as described above. β-actin served as a loading control. ^**p < 0.01; ^***p < 0.001 versus the scrambled shRNA (shCon) group, ^# p < 0.05, ^## p < 0.01, ^### p < 0.001 versus the ITPR3 shRNA group. CD44 OE: CD44 overexpression. shCon: Control shRNA