Fig. 1

Silencing HOXA10 inhibited LAD cell proliferation and promoted its apoptosis. a Expression of HOXA10 in LAD tissues (n = 42) and paracancerous tissues (n = 42) by RT-qPCR. b Immunohistochemical detection of HOXA10 in LAD tissues and paracancerous tissues (200 ×). c HOXA10 mRNA expression in HBE cells and 5 LAD cell lines (GLC-82, H1299, XWLC05, A549, and SPCA-1) by RT-qPCR, normalized to GAPDH. d HOXA10 protein expression in HBE cells and 5 LAD cell lines by Western blot analysis, normalized to GAPDH. e Expression of HOXA10 mRNA in response to different sh-HOXA10 determined by RT-qPCR, normalized to GAPDH. f Expression of HOXA10 protein in response to different sh-HOXA10 determined by Western blot analysis, normalized to GAPDH. g Cell viability detection by CCK-8. h The detection of apoptosis by flow cytometry. I, The cell cycle distribution through flow cytometry. j Cell migration by scratch test. k Cell invasion by Transwell assay (200 ×). * p < 0.05 vs. the paracancerous tissues (panels a and b), HBE cells (panels c and d) or A549 cells transfected with sh-NC (panels e-k). All experiments were done in triplicate