Table 4 Primer sequences for methylation-specific PCR
Gene | Primer sequence |
|---|---|
KLF4 (methylated) | F: 5′-CGTAGGGTTTAAATAGGTGATAACG-3′ |
R: 5′-AAATAATAAAAACTCGAACACCGAA-3′ | |
KLF4 (nonmethylated) | F: 5′-TGTAGGGTTTAAATAGGTGATAATGA-3′ |
R: 5′-AAATAATAAAAACTCAAACACCAAA-3′ |