Fig. 9

Hypoxia triggers a MAPKAPK5-AS1/PLAGL2/HIF-1α signaling loop. a Heat map of RNA-seq data exhibited the upregulation of MAPKAPK5-AS1 under hypoxia. b The upregulation of MAPKAPK5-AS1 and PLAGL2 induced by hypoxia was suppressed by HIF-1α knockdown. c The recognition motif of HIF-1α from JSAPAR database. d The hypoxia responsive element (HRE) in MAPKAPK5-AS1 promoter. In addition, the result of luciferase reporter assay is exhibited. e ChIP assay was conducted to validate the binding between HIF-1α and the HRE of the lncRNA MAPKAPK5-AS1 promoter under normoxia and hypoxia. f The regulatory effect of MAPKAPK5-AS1 on HIF-1α and PLAGL2 level. d The effect of PLAGL2 on HIF-1α and MAPKAPK5-AS1 expression under hypoxia. *p < 0.05