Fig. 2
From: Claudin-2 inhibits renal clear cell carcinoma progression by inhibiting YAP-activation
Claudin-2 expression is sharply downregulated in mouse model of spontaneous renal tumorigenesis: (a) Microarray analysis to determine mRNA expression changes in normal mouse kidney or KRas and β-catenin mutated mice kidney (γ-GTCreK-ras+G12D/Catnb+/Lox(ex3) with spontaneous tumor growth. RNA was extracted from the kidney of three individual mice from each group (two from KrasG12D/CatnbΔex3). Data shown for claudin-2 and claudin-14 expression; (b) Representative immunoblot and densitometric analysis of renal claudin-2 expression in γ-GTCre (WT), γ-GT/CreK-ras+G12D (activated K-ras in PTE cell), γ-GTCatnb+/Lox(Δex3)) (activated β-catenin in PTE cells) and γ-GT/CreK-ras+G12D/Catnb+/Lox(Δex3) (activated K-ras and β-catenin in PTE cells) (N = 2/group, except wild type); (c) Representative images of immunohistochemical determination of claudin-2 expression in normal mice kidney or mice with spontaneous renal tumors (y-GT/CreK-ras+G12D/Catnb+/Lox(Δex3). Data is presented as mean + sem. Statistical significance was determined by 1-way ANOVA and post hoc Tukey’s test for pairwise comparison. ****P < 0.0001. Scale bar = 50 μM