Fig. 5
RBM15 accelerates LSCC malignant progression by upregulating TMBIM6. a Western blot was performed to investigate the expression of RBM15 and TMBIM6 in AMC-HN-8 cells after transfection with shRBM15–2 and/or TMBIM6. b Western blot was conducted to examine the expression of RBM15 and TMBIM6 proteins in TU-212 cells after transfection with shTMBIM6–2 and/or RBM15. c Transwell assay was performed to measure the cell invasion ability of AMC-HN-8 cells after transfection with shRBM15–2 and/or TMBIM6. Hoechst staining assay was performed to measure cell apoptosis of AMC-HN-8 cells after transfection with shRBM15–2 and/or TMBIM6. d Transwell assay was performed to measure the cell invasion ability of TU-212 cells after transfection with shTMBIM6–2 and/or RBM15. Hoechst staining assay was performed to measure the cell apoptosis of TU-212 cells after transfection with shTMBIM6–2 and/or RBM15. e Knockdown of RBM15 inhibited TMBIM6-induced AMC-HN-8 cells subcutaneously tumor growth in nude mice. f Knockdown of TMBIM6 inhibited RBM15-induced TU-212 cells subcutaneously tumor growth in nude mice