Fig. 2

Silencing WDR3 suppresses the aggressive behavior of pancreatic cancer cells in vitro and pancreatic tumor growth in vivo. a and b RT-PCR (a) and western blot analyses (b) of WDR3 expression in PANC-1, MIA PaCa-2, and BxPC-3 cells infected with sh-Control or sh-WDR3s. GAPDH served as an internal reference. Data are presented as the mean ± SD of three independent experiments. Each sh-WDR3 group was compared with sh-Control group. Statistical analyses were performed with one-way ANOVA followed by Tukey’s multiple comparison’s tests. **, P < 0.01; ***, P < 0.001. C-E. PANC-1, MIA PaCa-2, and BxPC-3 cells were infected with sh-Control or sh-WDR3 #1. The cells were harvested for colony formation (c), MTS (d), and Transwell invasion assays (e) after 48 h of culture. Each bar represents the mean ± SD of three independent experiments. **, P < 0.01; ***, P < 0.001. F-H. PANC-1 cells infected with sh-Control or sh-WDR3 #1 were subcutaneously injected into nude mice. The tumors were harvested and photographed (f) on day 21. Data for tumor volume (g) and tumor mass (h) are shown as the mean ± SD (n = 5). Each sh-WDR3 group was compared with sh-Control group. Statistical analyses were performed with one-way ANOVA followed by Tukey’s multiple comparison’s tests. ***, P < 0.001