Fig. 4

WDR3 transcriptionally increases YAP1 expression in pancreatic cancer cells. a and b. Volcano plot (a) and heatmap (b) showing the differentially expressed genes in PANC-1 cells infected with si-Control or si-WDR3. The blue points represent the downregulated genes (n = 248), while the red points represent the upregulated genes (n = 242). c Heatmap showing a subset of WDR3 knockdown-regulated genes with a p-value < 0.1 participating in the Hippo signaling pathway in PANC-1 cells. si-WDR3 group was compared with si-Control group. Statistical analyses were performed with one-way ANOVA followed by Tukey’s multiple comparison’s tests. d and e RT-PCR analysis (d) and western blot analysis (e) to detect the mRNA and protein expression levels of YAP1 in pancreatic cancer cells infected with sh-Control or sh-WDR3s. GAPDH served as an internal reference. Data are shown as the mean ± SD (n = 3). Statistical analyses were performed with one-way ANOVA followed by Tukey’s multiple comparison’s tests. **, P < 0.01; ***, P < 0.001. F-G. RT-PCR analysis (f) and western blot analysis (g) to detect the mRNA and protein expression levels of YAP1 in pancreatic cancer cells infected with pcDNA3.1 or WDR3. GAPDH served as an internal reference. Data are shown as the mean ± SD (n = 3). Statistical analyses were performed with one-way ANOVA followed by Tukey’s multiple comparison’s tests. ***, P < 0.001. h RT-PCR analysis to detect the mRNA expression levels of CTGF and CYR61 in pancreatic cancer cells infected with sh-Control or sh-WDR3s. GAPDH served as an internal reference. Data are shown as the mean ± SD (n = 3). Statistical analyses were performed with one-way ANOVA followed by Tukey’s multiple comparison’s tests. ***, P < 0.001. i RT-PCR analysis to detect the mRNA expression levels of CTGF and CYR61 in pancreatic cancer cells infected with pcDNA3.1 or WDR3. GAPDH served as an internal reference. Data are shown as the mean ± SD (n = 3). Statistical analyses were performed with one-way ANOVA followed by Tukey’s multiple comparison’s tests. ***, P < 0.001