Fig. 6

The regulation of YAP1 induced by WDR3 was dependent on GATA4 in pancreatic cancer cells. A-B. LC-MS/MS identified an interaction between WDR3 and GATA4 (a) by detecting two peptides of GATA4 (b). c-d Coimmunoprecipitation showed the interaction between WDR3 and GATA4. e-f Western blot analysis showed the protein expression levels of specific genes. GAPDH served as an internal reference. g Western blot analysis to show the GATA4 expression in the nucleus and cytoplasm of pancreatic cancer cells infected with sh-Control or sh-WDR3