Fig. 1

Elevated ZNF561-AS1 positively regulates SRSF6 expression in CRC. a ZNF561-AS1 expression in CRC patients. Data from TCGA data resource. *p < 0.05. b ZNF561-AS1 expression is elevated in six patient CRC samples. *p < 0.05. c ZNF561-AS1 level is elevated in various CRC cell lines relative to CCD841 CoN normal human colonic epithelial cell line. n = 3, *p < 0.05. d ZNF561-AS1 expression was reduced by chemotherapy but restored in recurrent CRC samples. e SRSF6 expression in CRC patients. Data from TCGA data resource. f Positive correlation between ZNF561-AS1 and SRSF6 in CRC patients. Data from TCGA data resource. g Increased SRSF6 expression was observed in CRC patients tissue samples. h ZNF561-AS1 silencing suppressed SRSF6 mRNA levels in HCT-116 cells. n = 3, *p < 0.05. i ZNF561-AS1 overexpression elevated SRSF6 mRNA levels in HCT-116 cells. n = 3, *p < 0.05. j ZNF561-AS1 overexpression enhanced SRSF6 proteins level in HCT-116 cells. Actin was employed as loading control. Representative blots are shown (left panel). Ratios of level of SRSF6 vs Actin were computed after densitometric analysis of blot images on Image J (right panel). Data were from 3 independent experiments. k SRSF6 knockdown did not alter ZNF561-AS1 expression. n = 3, *p < 0.05. l SRSF6 overexpression did not alter ZNF561-AS1 expression. n = 3, *p < 0.05