Fig. 4

Induction of apoptosis by selinexor and doxorubicin in DDLPS models. a, Western blot analysis of survivin, p53, p21, and cleaved caspase 3 on tumors obtained from untreated (Ctrl) and selinexor- or doxorubicin-treated mice at different intervals from the end of treatment. The expression of autophagy (LC3B) and senescence (p16) markers was also assessed. A representative blot of three independent experiments is shown. For each protein, band intensity was quantified using Image J normalized to loading control reported below and referred to respective untreated control. The band intensity of LC3B-I and LC3B-II were quantified separately (above and below, respectively). b, Cleaved caspase-3 immunostaining of tumors obtained from untreated (Ctrl) and selinexor- or doxorubicin-treated mice at the end of treatments (upper panel) and quantification of the percentage of cleaved caspase-3 positive cells (lower panel). c, Flow cytometric assessment of TUNEL-positive cells after 72 h exposure to equimolar concentration of drugs in DDPLS cells (upper panel) and quantification of TUNEL-positive cells (lower panel). Results represent the mean values ± SD of 3 independent experiments. ^*P < 0.05, ^**P < 0.01, ^***P < 0.005