Fig. 5

Effects of selinexor on the expression and subcellular localization of survivin. Western blot analysis of survivin, MDM2, p53 and XPO1 in whole protein extracts (left panel) and fractionated protein extracts (right panel) of LS-GD-1 cells a, and LS-BZ-1 cells b, at different intervals of exposure to selinexor. c, Western blot analysis of STAT3 and acetyl-STAT3 expression on whole protein extracts (left panel) and survivin ubiquitination in protein nuclear fraction (right panel) of LS-BZ-1 cells at different intervals of exposure to selinexor. Representative blots of three independent experiments are shown. For each protein, band intensity was quantified using Image J normalized to loading control reported below and referred to respective untreated control