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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Circ-MBOAT2 knockdown represses tumor progression and glutamine catabolism by miR-433-3p/GOT1 axis in pancreatic cancer

Fig. 1

Circ-MBOAT2 was overexpressed in the tissues and cells of pancreatic cancer. a and b The expression level of circ-MBOAT2 was determined by qRT-PCR in 34 pairs of pancreatic cancer and paracancerous normal pancreatic tissues as well as HPDE, AsPC-1, BxPC-3, PANC-1 and SW1990 cells. c and d RNase R treatment assay was employed to illustrate the stability of circ-MBOAT2. e and f Cytoplasmic and nuclear circ-MBOAT2 analysis assay was performed to demonstrate that circ-MBOAT2 was mainly located in cytoplasm. The β-actin was employed for the normalization of circ-MBOAT2/MBOAT2/GAPDH, and U6 was used for U6. *P < 0.05, ***P < 0.001 and ****P < 0.0001

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