Fig. 5

Circ-MBOAT2 downregulation repressed pancreatic cancer progression by binding to miR-433-3p. a The effect of miR-433-3p inhibitors on miR-433-3p expression was presented by qRT-PCR in PANC-1 and SW1990 cells. b The impacts between circ-MBOAT2 silencing and miR-433-3p inhibitors on miR-433-3p expression were illustrated by qRT-PCR. c and d The impacts between circ-MBOAT2 repression and miR-433-3p inhibitors on cell viability were revealed by MTT assay. e The impacts between circ-MBOAT2 repression and miR-433-3p inhibitors on cell colony-forming ability were revealed by cell colony formation assay. f Flow cytometry analysis was employed to explain the effects between circ-MBOAT2 silencing and miR-433-3p inhibitors on the apoptosis of PANC-1 and SW1990 cells. g and h The influences between circ-MBOAT2 repression and miR-433-3p inhibitors on the invasion and migration of PANC-1 and SW1990 cells were disclosed by transwell invasion and wound-healing assays, respectively. i Glutamine assay kit was utilized to illustrate the effects between circ-MBOAT2 silencing and miR-433-3p inhibitors on glutamine consumption in PANC-1 and SW1990 cells. j α-KG assay kit was used to present the effects between circ-MBOAT2 silencing and miR-433-3p inhibitors on α-KG production. k Glutamate assay kit was utilized to illustrate the effects between circ-MBOAT2 silencing and miR-433-3p inhibitors on glutamate production in PANC-1 and SW1990 cells. The U6 and β-actin were employed for the normalization of miR-433-3p and circ-MBOAT2, respectively. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001