Fig. 2

CTD inhibits the migration and invasion of colon cancer cells. A wound-healing assay was performed to measure the effect of CTD on the migration of the CRC cell lines at 24 h. a Treatment with CTD remarkably inhibited the migratory ability of the CRC cells in a wound-healing assay. Scale bars, 100 μm. The representative images (up) and the summary bar chart (down) of the cells that migrated by ImageJ to calculate the area. b and c Transmembrane migration assay was performed to measure the effect of CTD at various concentrations on the migration and invasion of the CRC cells at 24 h. This result indicated that CTD suppresses migration and invasion. The data are shown as means ± SD of values from triplicate samples. (*p < 0.05, **p < 0.01, ***p < 0.001) indicate a significant difference compared to the control. d Protein expression of epithelial-mesenchymal transition (EMT) markers E-cadherin, N-cadherin, and vimentin levels were checked after CTD treatment and compared with control