Fig. 7

Correlative expression of YAP and HMGB1 are prognosis for clinical GBM. a Representative immunoblots of total lysates extracted from high grade samples probed with indicated antibodies. b & c The protein level of YAP was positively correlated with HMGB1 and LC3-II in gliomas. d Representative images of IHC staining of YAP and HMGB1 in clinical GBM samples. Scale bar: 25 μm. e The protein level of YAP was positively correlated with HMGB1 in gliomas examined by IHC. f-h Kaplan-Meier analyses for GBM patients with high or low level of YAP (f) or HMGB1 (g) or with YAP and HMGB1 both high or low expression (down of h). Correlations of IHC data for high or low YAP expression relative to level of HMGB1 (up of h). The cut-off to define high or low expression was the median value. i Working model: YAP promotes glioma autophagy and progression by promoting the transcription and translocation of HMGB1 from nucleus to cytoplasm, leading to autophagy