Fig. 6

ASS1 acquires tumor suppressor activity independently of arginine metabolism and the p53 pathway a The NO production in ASS1-upregulation or downregulation in HCC cells. b ASS1 and CHOP expression levels and c cell survival analysis in pCMV3 or ASS1-treansfected Huh7 cells with the NO scavenger treatment; cupral (left panel) and cPTIO (right panel). d Phosphorylation of H2AX (γ-H2AX) and p53 pathway related proteins expression level in pCMV3- or ASS-flag transfected p53 wild type cells (Fa2N-4 and HepG2 cells) and e Colony formation assay in pCMV3- or ASS1-flag transfected p53-wild type cells. f Expression of γ-H2AX p53 related proteins in p53-null cell (Hep3B) and p53-mutant cells (Huh7 and SNU475 cells). g Colony formation assay in pCMV3- or ASS1-flag transfected p53-null and p53-mutant cells. ^*P < 0.05, ^**P < 0.01 compared to control group. At least triplicate analysis in each set of experiment. ns; not significant, NO; nictic oxide